It could be a bit easier if your intended variable names matched those in the output.txt file. i.e.
$ cat Vars
while read LHS EQ RHS; do
case "$LHS" in
Freq|L) eval $LHS=\"$RHS\";;
esac
done < output.txt
echo Freq is $Freq
echo L is $L
$ ./Vars
Freq is 1900
L is 159
But even if they don't:
while read LHS EQ RHS; do
case "$LHS" in
Freq) F="$RHS";;
L) Len="$RHS";;
... etc
esac
done < output.txt